koch’s method with tuberculosis. 
125 
EXPERIMENTS WITH LUNG PLAGUE OF CATTLE. 
Inoculations with Sequestrum.—My next essay in this field 
was with lung plague, and my first experiment was made by 
diluting and inoculating the juice squeezed from a sequestrum 
from the lung of a cow which had been attacked at Orange, 
N. J., August 27th, 1881. The cow was killed January 30th, 
1882, and January 5th I inoculated three yearlings by injec¬ 
ting fprty drops into each lung of each animal, using a hypo¬ 
dermic syringe with long nozzle, which was passed through 
the intercostal spaces. Two of the yearlings coughed a few 
times, but all of them took to eating heartily. One, which 
may be called No. 1, was suffering from a chronic bronchial 
catarrh and one grain of the sequestrum was inserted into a 
subcutaneous pouch in front of its shoulder. As the time of 
inoculation all had a temperature of 103° F. For two days 
there was a very circumscribed crepitation in the seat of the 
inoculation, but on the third or fourth day this had disap¬ 
peared in all alike. The temperature of No. 1 remained at 
103° F.; that of Nos. 2 and 3 went down to 102° F. 
All remained without further change for six weeks, and on 
February 16th I again inoculated each in the right lung with 
forty drops of the slightly diluted juice of a sequestrum taken 
February 15th from a New York city cow by Dr. James D. 
Hopkins. This cow had been sick eleven weeks before her 
slaughter. Seventeen days later I found the temperature of 
Nos. 2 and 3 had risen to 103.5 Q F., and this it did occasion¬ 
ally for eight days, but there was no other indication of ill¬ 
ness. 
March 16th 1 inoculated these three and another as a test 
case, (No. 4.), in the right lungs, with the freshly exuded 
lymph from the lung of a cow which had sickened March 13th 
and was killed March 14th. March 23d, No. 4 coughed while 
temperature was being taken, and this was repeated daily 
until the 26th, when there was slight crepitus in the right 
lung. Temperature 103.75 P F., pulse 60, breathing quiet. 
Temperature on the 25th had been 103.5 0 , and by April 1st 
and 2nd had reached 105.75 0 F. There was slight dullness 
