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R. MIDDLETON. 
nent eradication of this disease. Should the Koch medium 
become a practicable means of determining tuberculosis in 
animals, it would not alone increase the efficiency of the 
sanitary police, but it would materially aid the development 
of the agricultural and breeding interests. 
To decide to what extent, and in what dose the agent 
reacted in cattle, the health office purchased two adult cows 
and one heifer. By special permission these were placed in 
an experiment stall in the Royal Veterinary College in Berlin, 
and at the expense of this institution were fed and attended. 
The trials were carried out by the government adviser, 
Roeckle, and Dr. Schiitz, rector of the college, under the di¬ 
rection of Prof. Dr. Koch. 
The two cows were selected as tuberculous, from a large 
dairy farm ; the calf was in perfect health, and was to serve 
as a physiological standard. All of the animals were examined 
at short regular intervals for several days previous, and sub¬ 
sequent to the injection; the chief purpose of this being to 
note the variations in respiration, temperature and pulse. 
At a determined time after the reception of the virus, the 
subjects were slaughtered. 
Each animal received 0.5 ccm. of a Koch fluid, diluted 
with 4.5 ccm. of a y 2 per cent, phenol solution ; the mixture was 
injected at one time upon the chest (dewlap) after the hair 
had been clipped and the spot cleaned and disinfected ; the 
puncture was afterward closed by a flock of absorbent cotton 
dipped in iodoform collodion. The results were satisfactory 
throughout and in no respect complicated. 
Case I.—Experiment animal; a seven year old cow of the 
Holland breed, 560 kilogrammes live weight; well advanced 
in pregnancy and yielding no milk. Bacillus tuberculosis not 
found in the expectorated secretion ; temperature, ioi q -io 2 ° ; 
pulse, 64 ; respiration, 18 per minute. Injection given at 8.30 
a. m. on the 24th of January ; the temperature steadily ad¬ 
vanced, with variations, up to 9 o’clock p. m., when it reached 
104° ; between 3 and 5 o’clock on the next morning it had 
registered 104.5 0 , by one o’clock p. m. it had receded to 102°. 
Post morten showed unequivocal tubercle of the lungs and 
