272 
R. MIDDLETON. 
II. Liver extract from sheep No. II. Mouse received 
one-tenth of the liquid, and after two minutes appeared ex¬ 
cited ; after four minutes the same jumped when the table 
was struck ; fifteen minutes after, there appeared light mnscle 
contractions; in nineteen minutes death supervened under 
clonic-tonic spasms. The second mouse was given two- 
tenths of the extract and in two minutes was excited ; four 
minutes after is, strange enough, quiet and immobile; six 
minutes after, eyes closed and seemed ill; ten minutes slight 
contraction ; sixteen minutes after, death under clonic-tonic 
convulsions. In both mice thirty seconds before death the tail 
was stiff and held upward, as though in opisthotonoid con¬ 
traction. 
III. Muscle extract sheep No. I. In six minutes after the 
reception of one-tenth of the fluid, the mouse showed some 
excitement. Second mouse received two-tenths of the same, 
and in seven minutes appeared frightened when the table 
was struck; in twenty minutes after both were easily fright¬ 
ened ; twenty-six minutes after, individual contractions ; forty- 
five minutes contractions progressing and great fear present; 
seventy-five minutes after, both animals crouched and appa¬ 
rently ill; in two hours after, both ate bread ; three hours, 
behave naturally, and are frisky; during five hours of con¬ 
tinued watching they maintain appearances of health, and on 
the next day showed themselves as well as previous t© the 
injections. 
IV. Muscle extract, sheep No. II. Animal received 
one-tenth of the extract, and in nine minutes died. The 
second mouse was given two-tenths and in eight minutes was 
also dead. 
V. The remaining muscle extract was mixed with 
30 ccm. of water and injected into a dog weighing ten 
kg. No sort of working could be differentiated, and after 
one hour the same was lively and free of any symptoms of a 
toxic nature. 
EXPERIMENT WITH ESERINE. 
A wether weighing thirty-two kg. received 0.5 grms. of 
eserinum sulphuricum ; death resulted in thirteen minutes. 
