432 
koch’s tuberculin. 
As to the quantity required to give a reaction, it will be 
seen that in case No. 3 1,000 milligrams were needed; while 
in case No. 4 a good reaction was obtained with 100 milli¬ 
grammes. 
Case No. i. —This case was a registered Guernsey calf, 
eight months old, condemned because of its having tuber¬ 
culosis and presented to the Commission for experimentation. 
The animal being young, strong and vigorous, and in the 
early stage of the disease, it was considered an exceptionally 
good case in which to test the curative properties of the 
remedy. The temperature of this animal was normal, as were 
also the pulse and respirations and, to all external appear¬ 
ances, it was a perfect animal. 
On April 3d, 1891, at 10 a.m., this animal was given a 
hypodermic injection of 100 mg. of tuberculin under careful 
antiseptic precaution; the temperature then registered ioif > 
five hours afterward it registered its highest point, I02-|°, this 
was at 3 p.m.; at 6 p.m. it had dropped to ioof° and after¬ 
wards fluctuated between ioo° and 102°. Not the slighest 
physical disturbance could be observed in this animal. These 
injections were made once a week until Ma} r 5th, and in no 
instance were there visible manifestations indicating the 
action of the remedy. May 5th the animal received 75 m §h 
of tuberculin, highest temperature I02^- Q ; May 6th received 
75 mg., temperature reached 103° ; May 7th, 75 tempera¬ 
ture reached 104° ; May 8th, 75 mg., temperature dropped to 
I02|°. 
For the next six weeks this animal received 75 tuber- 
culin daily. The temperature continued to gradually deciease 
until it remained stationary between ioi° and 102°. There was 
no disturbance of the economy other than the rise of tempeia- 
ture, and when repeated injections failed to have an influence 
in this respect, and the treatment had continued for over 
three months, the injections were discontinued. 
August 5th, the animal, while presenting no abnormal 
symptoms, was killed and an autopsy made, in order to ascer¬ 
tain the results of the treatment. Prior to death, physical 
examination revealed nothing but a very slight bionchitis. 
