KO0H*S TUBERCULIN. 485 
apparently tubercular. (The microscopical examinations of 
these tissues had not yet been completed-) 
Case No. 5.—A two-year-old Guernsey heifer in the early 
stage of tuberculosis (diagnosis made from the history of the 
case.) On the 9th of March injected 300 mg. of tuberculin. 
The temperature of the animal when inoculated was 100 
degrees ; five hours afterward the temperature began to rise, 
and in eight hours reached its highest point, 105-f 0 . It then 
began to fall and at the twentieth hour had dropped to 99 0 
degrees and afterwards fluctuated between ioi°and 102°. A 
second injection of 500 mg. was made on the 25th. This in¬ 
creased the temperature in twelve hours to io5f°. Twenty- 
four hours afterward it had dropped back to 99^. The 
autopsy examination showed a few small tubercles in the 
lungs, the latter showing evidences of having undergone 
retrograde change. Several spots of lobular pneumonia, the 
largest about the size of a man’s fist, were found in the central 
part of the posterior lobe of the left lung. The pleura pre¬ 
sented large masses of miliary tubercle in every stage of 
development, from the earliest to the calcareous degeneration. 
Pleuritic adhesions to diaphragm and to chest walls on both 
sides. Resting upon the sternum was a large mass of cheesy 
tubercle adherent to both diaphragm and pleura. 
The mediastinal glands were of enormous size and had 
undergone cheesy changes. The peritoneum presented a large 
number of tubercles at various points and in numerous stages 
of development; tubercular enteritis well marked. In the 
lumbar region, along the course of the blood vessels, were a 
number of dark red tubercles, which, when cut, exuded a dark 
fluid. Probably this was one of the earliest stages of tuber¬ 
cular formation. The liver, spleen and kidnej^s were appar¬ 
ently normal. 
Case No. 6 . —Was a black cow five years old, well advanced 
in tuberculosis. At the time of injection, and for several days 
previous, the animal was very much depressed, pulse not per¬ 
ceptible at points where it is usually taken, extremities cold, 
respiration thirty-eight per minute and labored. Head ex¬ 
tended ; animal very weak and in a generally distressed con- 
