460 
EXPERIMENTAL PATHOLOGY. 
of .—io° F. During the ten months it was left there, it was 
exposed to temperature varying between .— io° and .—20°. 
especially in September, 1890, December, 1890 and March, 
1891. On the first of June the rabbit was taken from the 
cool chamber, conveyed in an elevated temperature upon a 
journey that lasted five hours, after which he was entirely 
thawed out. He was then again placed on ice and the next 
day the extraction of the spinal cord was performed, to 
test if the rabic virus had resisted the prolonged action of 
cold. 
With the bulb, which appeared like that of a rabbit just 
killed, perhaps of less consistency, a large and strong rabbit 
was inoculated by trephining. On the fourteenth day he 
was sick, and died paralytic on the sixteenth day. With its 
bulb five other rabbits were inoculated, through the anterior 
chamber of the eye : one died the next day from traumatism 
foreign to the operation, the four others succumbed with all 
the symptoms of paralytic rabies. The bulb of one of these 
animals was used to inoculate two other rabbits through the 
eyes ; both died with rabies. 
To make the experiment more positive, two rabbits were 
inoculated with the bulbs of these last animals ; each rabbit 
received one inoculation from one bulb only. These also died 
with rabies. 
Conclusions :—With the four successive series of inocu¬ 
lations, rabies has always taken place and “ it seems evident 
that cold has no action upon rabic virus J 
Mr. Laquerriere has already proved that the virus of 
pleura-pneumonia can be kept for a long time when exposed 
to intense cold. If the same can be said of the vaccine for 
rabies or other vaccine fluids, the work of the preservation of 
prepared vaccine will be considerably simplified. The results 
of the experiments obtained by Mr. Jobert seem to indicate 
that this mode of preservation is possible.— Jour . Soc. Scientif. 
EARLY DIAGNOSIS OF BOVINE TUBERCULOSIS BY EXAMINATION 
OF THE OCULAR HUMORS. 
Mr. L. Mandereau has made numerous investigations in 
