498 
&. MIDDLEfON. 
the direct result of personal and accidental inoculation ; his 
experiments consequently remain incomplete. 
I projected a number of similar investigations with lymph 
which I cultivated, and will rehearse my results in the follow- 
ing paragraphs: 
The fact that the surface of the potato,—upon which the 
breeding of the bacillus was affected,—was at first green, later 
brown, and finally of a black color, leads me to suppose that 
this small organism generates a product which colors the soil 
upon which it grows and propagates. 1 his must be at once 
a peculiar and a specific material, since no other bacterial or¬ 
ganism is able to eause a similar play of tints upon the potato 
section; old and hard potato cultures of the malleus bacillus 
are a jet black color. 
By pouring over this a liquid of equal parts of water and 
glycerine, and placing the latter in an apparatus maintaining 
a temperature of 95 ° F., it is possible to obtain an extract 
which, after being filtered several times, and sterilized by 
steam, possesses a characteristic odor, neutral or slightly acid 
reaction, a dark opaque yellow color and oily consistency. 
This extract contains the coloring matter of the bacillary col¬ 
ony, while the insoluble products of the organism remain 
upon the tuber. 
An extract or concentration prepared in such a manner is 
called “ malleine ” after receiving a dilution of a few drops of 
corrosive sublimate solution. On April 16, I injected 0.2 
ccm. into each of two guinea pigs; one was healthy, the other 
had been infected with glanders on April 1. I also inocu¬ 
lated another pig on the latter date, but did not afterward 
inject malleine. 
The sound subject never manifested any reaction referable 
to the inoculation, but it died a few days later of an acquired 
disease. Some time later 1 gave the affected pig another in¬ 
jection of 0.2 ccm. The abscesses in the inguinal region here¬ 
upon rapidly disappeared, but there developed a suppurative 
inflammation of the left eye. The animal is living to this 
dav. The second animal infected by glanders, but not receiv¬ 
ing the glanders lymph, developed abscesses in the inguina 
