500 
R. MIDDLETON* 
No. 5. Brown mare, one and one-quarter years old, well 
nourished, no external signs. 
No. 6. Brown filly, fourteen days old. 
In Nos. 1, 2, 3, and 4, I used 3 ccm. of the fluid, i. e. 0.3 
ccm. of glanders lymph ; on No. 5, 0.2 ccm. of lymph, and on 
No. 6,0.1 ccm. of lymph; the injections were made hypo¬ 
dermically on the neck or shoulder by means of a sterilized 
syringe. The first puncture occurred at two o’clock P. M. 
and was followed at 9.30 P. M. by a second puncture of 0.5 
ccm. malleine in Nos. 2, 3, and 4, and of 0.3 ccm. in Nos. 1, 5 
and 6. The suffixed table shows variations of temperature 
and pulse rate : (See page 501.) 
Soon after the inoculation there developed at the point 
of injection a sensitive, doughy and variable swelling of the 
cutis and subcutis in all animals excepting the sixth. 
Considerable vacillation of temperature was induced, as 
will be seen from the table in Nos. 1, 2, 3, 4 and 5 ; this was 
most intense fifteen hours subsequent to the first and eight 
hours to the second injection. In No. 1 the temperature 
advanced from ioi° F. to 104° F.; in No. 3 the increase 
amounted to 3*9^° in the others fiom 2.5 F. to 3*4 
F. In No. 6 a slight increase of 0.9 0 F. occurred seven hours 
after the first injection ; but not only did it not rise after the 
second injection, but it actually fell to 100 F. While the 
five horses were weak and apathetic on the nth of June, the 
foal experienced neither of these two states ; the appetite of 
all six horses remained unaffected after the inoculations. 
Nos. 1 and 4 exhibited nasal discharges; the former of 
mucoid nature and from the left nostril—the latter watery 
and bilateral. The animals were destroyed at 10 A.M. June 
1 ith ; glanderous changes were present in Nos. 1, 2, 3, 4 and 
5, while in No. 6 no alterations,, either nodular or ulcerous, 
were apparent. The most considerable pathological lesions 
were found in Nos. 2 and 3 ; these consisted of fresh red 
granules and old caseous and calcareous nodules, together 
with a chronic pneumonia with numerous cicatrices in the 
trachea and larynx. In Nos. 1, 4 and 5 the pulmonary lesions 
were more circumscribed, but otherwise the same. Imme- 
