718 
S. WHITBECK. 
agar being substituted for the gelatin in making agar-agar. 
After the above preparations each media was heated to the 
boiling point, filtered into flasks, sterilized for three subse¬ 
quent days in a steam sterilizer, then placed in test tubes 
previously plugged with cotton and sterilized in dry sterilizer 
at 130° C. Each tube was filled to about one-fourth its ca¬ 
pacity and all again sterilized in the steam sterilizer at ioo° 
C. 
The laboratory work consisted in growing the several 
germs in the differant media and inoculation experiments with 
mice and rats. 
Staphylococcus pyogenes var. aureus. —August 7, 
inoculated tube of gelatin from a boil on student’s wrist. 
August 8, no growth discernable. August 9, a small white 
growth on surface. August 10, gelatin hall liquefied and the 
growth adhering to side of tube, of a yellowish color ; made a 
microscopical examination and found the germ to be a small 
organism arranged in groups. August 12, gelatin entirely 
liquefied. A tube of agar-agar was inoculated. August 13, 
a very small white growth had developed on the surface. 
August 14, growth as large again and slightly yellow, with a 
flaky white growth along track of needle. August 15, one 
half surface of agar covered with a deep yellow growth. On 
August 19, a tube of bouillon was inoculated. August. 20, 
bouillon quite turbid and a slight sediment of a yellow color, 
more growth on agar-agar. Inoculated a rat over root of tail 
from agar-agar culture. August 21, abscess at seat of inocula¬ 
tion, from which the same germ previously mentioned was 
isolated. August 22, rat died in forenoon ; examination showed 
the presence of a small organism in the liver, an inoculation 
from liver in agar-agar developed an impure culture from 
which the aureus was isolated. Two mice were inoculated 
on the 25th, one yielding the same results produced on rat, 
and other an abscess at seat of inoculation. On the 27th, inoc¬ 
ulated sterilized potato in tube. August 29, a small yellow 
growth at point of inoculation. August 31, growth extended 
over entire surface of potato and of a deep yellow color. 
Conclusions .—A germ of suppuration, and when gaining 
