A STUDY OF THE MICROBES OF PUS. 
7‘Zl 
but little larger, with a few granular points surrounding the 
main growth. September 15, inoculated a tube of gelatin. 
September 16, a small granular growth along track of needle 
and a white growth one-fourth inch in diameter on surface. 
Microscopical examination showed the germ to be a short 
bacillus, somewhat shorter than the pyocyaneus. September 
18, inoculated potato in tube. September 19, a white growth 
at point of inoculation, slowly spreading over the entire sur¬ 
face. September 18, inoculated bouillon. September 19, 
bouillon slightly turbid, small white colonies throughout the 
media. Inoculated a rat from agar-agar culture. September 
20, a small abscess at seat of inoculation. In the afternoon 
the rat died. A culture from the abscess taken before the 
rat died showed bacillus of various lengths. From this the 
short bacillus was isolated by means of a roll tube agar-agar, 
and grown on the different media with the same results as 
mentioned above. 
No internal lesions were presented on post-mortem exam¬ 
ination, though a culture from the liver produced a white 
growth on agar-agar that developed very slowly. Microscop¬ 
ical examination showed a short bacillus having the same 
dimensions as the organisms used in inoculating. September 
25, inoculated a mouse, which died on the fifth day after in¬ 
oculation. Examination showed micrococci and a short 
bacillus in the liver, which I failed to isolate. 
Conclusions .—Probably a pathogenic and mnoerobic organ¬ 
ism in nature. 
Bacillus. —September 10, made Esmarsch tube of agar- 
agar from abscess produced by hypodermic injection of chlo¬ 
ral hydrate under skin of mare affected with tetanus. Sep¬ 
tember 12, numerous white growths had developed on the 
surface of media. Cover glass preparations of these growths 
under the microscope, with a one-twelfth oil immersion objec¬ 
tive, showed an organism nearly circular in outline. Under 
the one-sixteenth, however, some of the germs appeared to 
be a little elongated and stained at polar ends. Inoculations 
were made in agar-agar and gelatin. September 13, a whit¬ 
ish, very nearly transparent, growth had developed at point 
