722 
S. WHITBECK. 
of inoculation in each media. September 14, growth extend¬ 
ed over entire upper surface of agar-agar and gelatin, and 
clear white in color. Inoculated bouillon from agar-agar cul¬ 
ture. September 15, bouillon quite turbid with a clear white 
sediment in bottom of tube. Microscopic examination of this 
one-day-old culture showed its spore formation to be taking 
place very actively. September 16, inoculated potato. Sep¬ 
tember 17, a white stringy growth developed over the upper 
surface. Three mice and lour rats were inoculated with this 
germ in the course of my studies; one of the mice only be¬ 
came affected, and died the fourth day after inoculation. 
Microscopical examination of agar-agar cultures from the 
liver and spleen showed among others a very short bacillus 
stained at the polar ends similar to the germ under observa¬ 
tion. No further results were obtained from inoculations 
with this organism. 
Pink Bacillus, —September 1, inoculated gelatin from an 
abscess on horse’s wither. September 2, a bright pink growth 
developed at point of inoculation. September 3, growth 
about one-fourth inch in diameter, and having a circular out¬ 
line. Microscopical examination showed a bacillus about 
twice as long as the pyocyaneus. Inoculated gelatin in tube. 
By the 7th a similar growth to that on agar-agar had devel¬ 
oped. Three mice were inoculated with this germ without 
any result. 
Conclusions. — An asrobic organism and saphrophytic. 
To summarize from the above experiments and observa¬ 
tions Bacillus pyocyaneus proved to be the most malignant 
germ of those isolated, while the Staphylococcus pyogenes aiire- 
us was most often found, and in each case in originally closed 
abscesses, thus proving that the infection was from the blood, 
or from the tissue at seat of abscess. 
In conclusion, I wish to express my thanks to Dr. W. B. 
Niles and Mrs. Niles for faithfully preparing water-colored 
sketches of the germ isolated, also of the growth of each germ 
on agar-agar, and to Prof. Pummel for the kindly services 
rendered in my laboratory work and the preparation of this 
thesis. 
