14 
W. L. WILLIAMS. 
of the mallien test, the animal is destroyed after having - been 
appraised. 
The reaction or symptoms produced by mallein in glandered 
animals are well marked and highly characteristic. Preparatory 
to the test the temperature of the suspected animal is taken two 
or three times during the day prior to the trial, and a record of 
the temperature is again made at the time of injecting the mallein. 
The proper dose of mallein is then injected beneath the skin, 
usually along the neck, by means of an inoculating or hypoder¬ 
mic syringe. The spot where the injection is made is first 
cleansed by washing with a solution of corrosive sublimate or 
other anteseptic liquid and the needle of the syringe is carefully 
disinfected by immersing in such a solution or holding in a hot 
flame for a moment, until any forms of bacterial life which may 
exist in the needle are safely destroyed. These precautions are 
taken to render it certain that nothing but the pure mallein is 
introduced beneath the skin thus avoiding the danger of compli¬ 
cating or rendering valueless the trial, Subsequent to the in¬ 
jection the temperature of the animal is taken every two hours 
for twenty-four hours, and careful record is kept of each meas¬ 
urement. 
In animals suffering from glanders even in a mild degree, 
showing no outward signs, the temperature usually shows no 
particular change after the injection until after a lapse of five to 
eight hours and sometimes longer, when it suddenly rises as 
much as two, three or more degrees centigrade, above the nor¬ 
mal body temperature, the highest point being usually reached 
eight to sixteen hours and sometimes later after the injection, 
after which the temperature slowly falls, reaching the normal 
again in twenty-four to thirty-six hours, or longer. 
At the point where the mallein is injected the glandered 
animal usually shows a very considerable painful swelling, in 
many cases the animal becomes chilly as shown by shivering, 
the general appearance dull, the hair rough, appetite is lost, and 
in some cases any existing symptoms of glanders are aggra¬ 
vated, the nasal discharge if present before, may become in- 
