58 
GEORGE N. KINNELL. 
temperature and were found very extensively diseased, while 
cow 5 did not respond either so quickly or so much and yet 
was only slightly diseased. 
Again cows I, 2 and 4 did not respond at all to a 
small dose, but when tested with a large dose they responded 
quickly and very decidedly, yet the lesions were but slight and 
in every case were of long standing. Cow 4 was fourteen 
years old. The bronchial glands in this cow and in cow 1, 
were enlarged to the size of a hen’s egg, the caseous pus they 
contained was very much dried up, and in the case of cow one 
was so thoroughly impregnated with calcareous deposit as to 
make it a difficult matter to cut it with a sharp knife. 
There is one clinical feature to be noticed in testing with 
small doses, a feature which was pointed out to me by Dr. 
Cooper Curtice, who examined all my small dose charts, and 
that is that the reaction does not set in so early by four or five 
hours as it does when larger doses are used. Consequently it is 
necessary to continue taking temperatures four or five hours 
longer than is ordinarily required when the larger dose is used. 
There is one case of an animal very badly diseased which 
did not react to a small dose, but this creature had twice previ¬ 
ously been tested with full doses, first with a tuberculin prepar¬ 
ation sold by Metcalf, a few weeks later with 2 cc. Bureau tubercu¬ 
lin and five days later with one mimim of Koch’s tuberculin. It 
was noticed that after each of the first two tests the animal was 
much prostrated, the breathing accelerated and the appetite im¬ 
paired, from which she took from a week to ten days to recover. 
I am frequently asked to explain how I first came to use 
such an unusually small dose of tuberculin. The discovery was 
the result of a sheer accident. In the January, 1894, number of 
the Veterinary Magazine , there is an original article by Dr. A. 
E. Conrow, entitled “ Tuberculin as a Diagnostic Agent.” He 
says his method is similar to that used by Dr. Pearson. After 
details as to the preparation of tuberculin, he says: “Then with 
sterilized hypodermic syringe I inject 0.2 to 0.35 cc. of the ten 
per cent, solution regulating the amount by the size and condi- 
