PRODUCTION OF PERIPNEUMONIA CONTAGIOSA. 
259 
If I add that the colonies where the germs are more numer¬ 
ous are liquefying, it will be logical to conclude that the 
pneumobacillus liquefaciens is the pathogenic agent of pen- 
pneumonia. 
However, as this mode of demonstration might yet be only 
an indirect solution to the second question of this paper, I 
will not further insist, as I am prepared to give a positive an¬ 
swer, as follows: 
First, I was convinced that the effects of my inoculations 
to demonstrate the etiological part of the pneumobacillus 
had proved insufficient because of the activity of the mi¬ 
crobe grown in my cultures having originally been too weak. 
And again, I have observed, as early as 1889, that the organ¬ 
isms'contained in the pulmonary serosity had become more 
virulent while growing under the skin of cattle. I was there¬ 
fore justified in hoping to find more active microbes, such 
as I desire them, in the caudal.lesions following. Some trust 
the inoculation by Willems’ method, lesions gradually in¬ 
creasing notwithstanding the bad surrounding conditions. 1 
was anxious to undertake a new series of cultures and of ob¬ 
servations. Mr. Robcis, sanitary veterinarian, of Pans, gave 
me the opportunity. # . 
In the deep layer of the dermis and in the intermuscular 
connective tissue of a caudal region tumefied under the in¬ 
fluence of a peripneumonic virus, I found a great many more 
microbes than in the centre of pulmonary lesions. These mi¬ 
crobes multiplied rapidly and abundantly in beef bouillon 
strongly peptonized. . 
By Koch’s method for the isolation of the species I sep¬ 
arated from the cultures two bacilli differing principally from 
each other by the power of one to rapidly liquefy gelatine. 
The non-liquefying bacillus is generally shorter than the 
other. Both are abundantly provided with ciliae. The lique¬ 
fying bacillus answers exactly to the characters that 1 found 
long & ago in the pneumobacillus. In cultivating pure I was 
enabled to experiment on its physiological action. 
First I had been surprised at the intensity of the sub¬ 
cutaneous effects of 2 c.c. of a second generation-effects 
