260 
M. S. ARLOING. 
that were quick but exactly similar to those of the most ac¬ 
tive pulmonary serosity. I thought the opportunity excel¬ 
lent to try to obtain fructuous nitro-pulmonary inoculations. 
I prepared a culture of third generation, and the next 
day I pushed 2 c.c. in the thickness of the right lung of a 
fifteen months bull. This was repeated the next day. Three 
days later the animal was killed. At the post mortem I found 
a pneumonic mass of the size of the wrist, presenting to the 
highest degree all the characters of the lesions of the natural 
contagion. 
In a second experiment I inoculated in the lung of a 
young bovine a culture of fourth generation, with the small 
dose of o c.c. 5, to avoid the immediate toxic effects of large 
doses, and renewed the inoculation for three days with cul¬ 
tures of following successive generations. Six days after 
the first inoculation the animal was killed. The post mortem 
showed well characterized pneumonic centers and pleuritic 
lesions opposite them, as well as at the antero-inferior part of 
the chest. 
In a third experiment, to still better avoid the immediate 
toxic effects, I simply injected the microbes contained in 
10 c.c. of a tenth generation culture. The injection having 
been introduced a little too far back, the bacilli were by 
chance deposited beyond the lungs, on both faces of the dia¬ 
phragm. At the post mortem made five days later I found 
a sero-fibrinous thickening of the diaphragm, extending to 
the lung in front, to the peritoneum and the liver behind, in 
such a way that the three organs formed the center of a tumor 
surrounded with false membranes. I found, besides, a well- 
marked pleurisy on a level with the pericardium and the two 
anterior lobes of the lung, citrine effusion in the pleura, en¬ 
largement of the mediastinal and sub-pleural lymphatic 
glands. 
conclude, I have reproduced in the bovine, with 
pure cultures of the pneumobacillus taken between the sec¬ 
ond and the tenth generations, the typical alterations pro¬ 
duced under the skin or in the chest by the virus of the peri- 
pneumonic contagiosa. I then peremptorily can say, first, 
