422 
W. 8ILBERSCHM1DT. 
The planting of the blood or of the culture thus heated 
remains sterile, and the injection of small doses of this liquid 
(|- cc.) produces no trouble in the rabbit. But by opposition, 
the injection of larger doses is not so harmless; it may pro¬ 
duce death by intoxication. 
Let us consider, first, the toxine of swine plague, to which 
1 gave special attention. My experiments justify me to en¬ 
tirely endorse those of Selander; but as I have not injected 
as large quantities of toxic blood as he did, I have not to 
record as rapid a case of death. An intravenous injection of 
2 to 4 cc. of blood kills the rabbit in from 12 to 24 hours. 
There are individual differences; while the virus kills surely 
in a given time, some animals resist better than others the ac¬ 
tion of toxine. 
Two kinds of intoxication must be recognized—the acute 
and the chronic. In the first, one observes the same morbid 
symptoms following the injection of virus; the latter, after a 
variable time, produces death by cachexia. 
For swine plague the results have been noticeably the same, 
no matter what liquid was injected : heated blood, blood ster¬ 
ilized with thymic acid or formic aldehyde, bouillon cultures 
or those of emulsionized gelosis in bouillon and sterilized by 
heat; or, again, cultures free from their microbes by filtration 
through the Pasteur bougie. 
The quantities injected have varied between ^ and 10 cc. 
The cultures were supported in a larger dose than the blood, 
and contained less toxine. And, again, blood sterilized with 
thymic acid has shown itself more toxic than heated blood, 
and it has seemed to me that the repeated or longer action of 
I 
1 
1 
i! 
i 
heat diminished the toxic power. 
The largest number of intoxicated rabbits died with ca¬ 
chexia. Quantities of toxic liquid not exceeding 1 cc. were 
generally well tolerated ; in one case, however, 1 cc. of blood 
has produced death in 20 hours. The injection of 2 cc. of 
blood, or of 3 to 5 cc. of sterilized culture, produced a notice¬ 
able diminution in the weight, varying from a quarter to a 
third of that of the general weight of the rabbit. The maxi¬ 
mum of this diminution is observed after a few days (1 to 3) 
