424 
W. STLBERSCHMIDT. 
No. 339.—ik-610 ; subcutaneous injection of 4 cc. of the same culture sterilized 
at 120 0 during five minutes; the next day the rabbit weighs ik-440, the 5th ik-335, 
the nth ik-125 ; he is moribund and observed no longer. 
The injections that are not specified were made in the 
marginal vein of the ear. 
In a few cases I have added from 2 to 6 drops of Gram 
solution to the liquid to be injected, with the object to have 
a vaccination more efficacious, but it was not so; small quan¬ 
tities of iodine are well tolerated. 
For hog cholera , the results are analogous; but, as in the 
beginning, the microbes did not possess a constant virulency ; 
it has happened that some rabbits supported large doses of 
blood or of sterilized culture, while others exhibited serious 
symptoms with small quantities of toxine. The sterilization 
with the thymic acid was longer and less certain than for 
swine plague. Here are some experiments : 
No. 218.—Intravenous injection of 4 cc. of diluted blood (2 cc. nearly of pure 
blood) sterilized with thymic acid ; death in 36 hours ; blood and cultures sterile. 
No. 262.—Injection of 2\ cc. diluted blood sterilized with thymic acid ; death in 
about 12 hours ; blood and cultures sterile. 
No. 140.—2k-i6o; injection of 3 cc. of culture heated at 58° ; death in 2\ days; 
reduction of weight of 450 grammes; blood and cultures sterile. 
No. 131.—ik-810; injection of f cc. of blood sterilized by heat; the 3d day 
ik-540, the 12th ik-690, the 2ist ik-840. 
No. 268.—ik-720; injection of % cc. of blood sterilized by contact with thymic 
acid; the next day a second injection of 1 cc.; great reduction of weight; the 3d 
day ik-515, the 6th ik-400, the 8th ik-375, the 16th ik-415, the 21st ik-480, the 
28th ik-650, and the 34th ik-Soo. 
No. 146.—ik-640 ; injection of 2 cc. of culture on gelosis diluted in bouillon and 
sterilized. Initial weight the 8th day; 28 days after the first injection the rabbit re¬ 
ceives 2j cc. of culture also sterilized by heat; the day after he weighs ik-535 ; 8 days 
later ik-450 ; a third injection made ten days after the second brings the weight down 
to ik-450, and the animal requires more than a month (40 days) to recover his for¬ 
mer weight. 
No. 254.—ik-700; injection of 1 cc. of blood sterilized with thymic acid ; the 
third day ik-860 ; second injection of 1 cc. with a slight reduction of weight, ik-760; 
a third injection of the same blood is made eight days later (weight ik-835) ; the 
third day after, ik-600 ; this weight remains, and the rabbit dies nine days after the 
last inoculation. Small spleen ; blood and cultures sterile. 
The two experiments last described show how little the 
organism gets accustomed to the toxine. We can resume the 
results in the following propositions: 
