426 
W. 8ILBER8CHMIDT. 
3d.—Cultures filtrated with Pasteur bougie. 
Besides, I have obtained partial, though evident, results 
with— 
4th.—Cultures sterilized at 120°. 
5th.—Blood of vaccinated animals. 
Following are some examples in support of these data: 
No. 9.—Weight the 28-IV, ik-575 ; four injections of 5^ cc. of diluted blood 
sterilized at 58° ; the 28-V, a month after the first injection, he receives cc. of 1 
virulent culture. The witness dies in less than 24 hours. 
Second injection of trial the 6th-VI; the witness dies also in less than 24 hours. 
The vaccinated rabbit, after a few days, has an abscess at the point of inoculation, 
treated by incision and cauterization with the red cautery. 
Third trial the 26-VII with similar result, though one month and a half has 
elapsed since the last injection ; the witness dies. In these two months the subject 
has gained 480 grammes. 
a No. 54.—Weight the 26-VII, ik-835 ; four injections, in all 5^ cc. of blood ster¬ 
ilized by heat. The first trial takes place the 18-VIII, two days after the fourth 
injection; the witness dies in less than 16 hours; the vaccinated animal resists. 
After being injected again, in three sittings, 2 \ cc. of heated blood, he is submitted 
to another trial the 28-X and resists it, having, it is true, an abscess that I cauterize 
12 days later ; he weighed then 2ks-250—an increase of 400 grammes. 
No. 25.—Weight the 3d-IV, ik-730; six injections, altogether 8 cc. of bouillon 
culture and gelosed culture diluted in sterilized bouillon ; the injections are made far 
apart at first, on account of the reduction in weight. First trial the 17-XII, 13 days 
after the last injection of toxine ; the rabbit weighs 2ks-450 ; the witness dies in less 
than 18 hours ; two other trials are equally well tolerated. 
No. 224.—Weight the 2-III, ik-960; four injections, altogether 18 cc. of bouil¬ 
lon cultures sterilized at 58 to 6o° ; first trial the 29-IV, the rabbit has a small 
abscess which opens of itself; the witness dies in less than 10 hours ; three new trials 
the 17-V, 10-VI and 14-VI. 
No. 183.—Weight 17-I, ik-710 ; three injections, altogether 5^ cc. of bouillon 
culture filtrated with Pasteur bougie ; the rabbit stands two trials the 19-II and the 
14-III, after receiving 2 \ cc. of filtrated culture the 2-III. The 23-III he weighed 
ik-925. 
No. 197.—Weight the 29-I, ik-600; four injections, altogether 8 cc. of diluted 
blood (about half of water) sterilized with thymic acid ; trial the 10-III ; the witness 
dies in less than 16 hours; two other trials the 19-IV and 2-V, with witness in the 
last. The 17-V the rabbit weighs 2ks-300. 
No. 198.—Weight the 29-I, ik-620; three injections, altogether 9 cc. of diluted 
blood sterilized with thymic acid ; three trials the 19—II, 27-II and 7—III ; the wit¬ 
ness injected on the third trial dies in less than 10 hours. The rabbit weighs 2ks-ioo 
the 13—III. 
No. 195.—Weight the 27-I, 2ks; injection of 12 cc. of culture heated at 120 0 
during five minutes; the rabbit has regained his initial weight the 26 -II; he is, like 
most of the rabbits to test, injected with ^ cc. of yirulent culture under the skin of 
