428 
W. SILBERSCHMIDT. 
No. 314.—Weight the 21-V, ik-720; six injections, in all 6J cc. of sterilized 
blood at 58°. Test injection, made the 13-VI, kills the witness in 18 hours, and gives 
rise in the vaccinated rabbit to a small abscess, which remains localized. 
Vaccination against hog cholera is possible, and takes place 
as that of swine plague. The cultures have not given me good 
results, as they were not virulent enough, and probably did 
not contain, after sterilization, enough toxines. 
To resume, rabbits are liable to be vaccinated against swine 
plague and hog cholera. For the former I have obtained— 
thanks to the virulency of the bacillus—good vaccination by 
repeated injections of sterilized blood and of cultures heated 
at 58° or filtrated. The injected quantity each time must be 
weak, 1 cc. and even less for the blood, \ cc. for the cultures. 
These doses are supported without sensible symptoms, and it 
is possible to have the injections repeated at short intervals, 
and to thus obtain a rapid immunity against the virus. If, on 
the contrary, one injects a too large quantity of toxine, the 
animal reduces considerably in weight, often dies, and if it 
resists, requires a long time to recuperate to its initial weight. 
And besides, the degree of vaccination does not depend di¬ 
rectly on the absolute quantity of injected toxine, as it was 
proved by several observations; rabbits were vaccinated in 
ten days by a daily injection of ^ to cc. of sterilized blood, 
when the same quantity, injected at once, gave rise to great 
reduction in flesh and failed to vaccinate. Before injecting 
another dose, one must wait until the animal has regained its 
initial weight, or he runs the risk of seeing him die, or at all 
events to see him lose still more flesh; also the vaccination 
takes longer to be perfect. As a general rule, rabbits have 
stood the test inoculation after having received 3 to 5 cc. of 
sterile blood; for the cultures, the necessary quantity to vac¬ 
cinate is greater, and varies in proportions, though in two 
cases 7 cc. proved sufficient. It is clearly evident that quan¬ 
tities of cultures heated at ioo° and 120° must be much larger 
to produce the same effect. To the point of view of the vac- 
cinatiou of the rabbit, there are, then, only differences of 
quantity between hog cholera and swine plague. 
It remains for me to speak of the vaccination of a dog 
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