492 
S. J. J. HARGER. 
25th day, injection of 3 cc. pure toxin, slight oedema, no 
fever. 
28th day, injection of 5 cc. pure toxin; slight oedema, no 
fever. 
30th, 32d and 36th days, injection of 5 cc. pure toxin; slight 
oedema, no fever. 
39th and 41st days, injection of 10 cc. of pure toxin; slight 
oedema, no fever. 
43^, 46th, 48th and 50th days, injection of 30 cc. pure 
toxin, oedema marked, disappearing in forty-eight hours. 
53d, 57th, 63rd, 65th and 67th days, injection of 60 cc. pure 
toxin , oedema marked, disappearing in forty-eight hours. 
72d day, injection of 90 cc. pure toxin; oedema marked* 
disappearing in forty-eight hours. 
80th day, injection of 250 cc. pure toxin; oedema marked, 
disappearing in forty-eight hours. 
TETANUS. 
The toxin of tetanus is very active. A healthy horse has 
been destroyed by £ cc. (2 drops). Horses can be immunized 
against tetanus in the same manner as against diphtheria. The 
toxin pure, heated to 65° C. or mixed with iodine is injected 
in repeated and gradually increasing doses at intervals. The 
animal thus becomes so “accustomed” to the poison that 250 
to 300 cc. can be given at a single injection, enough to destroy 
2,500 horses (Nocard). 
This serum is preventive and curative; it can be used as 
a vaccine or a therapeutic agent. Injected under the skin of 
a tetanic subject it is efficacious only in proportion as the time 
elapsed since the infection has been short. I have no statis¬ 
tics of this treatment in a horse, but hope to be able to give it 
a trial. It is useful as a preventive only when the virus, or 
shortly afterwards, is introduced into the circulation. Unlike 
diphtheria, tetanus is not recognized until the system is satu¬ 
rated with the poison, then no treatment is of much avail. 
The serum may be recommended as a preventive in places 
where tetanus is almost epizootic, or in certain operations and 
wounds of the extremities which are frequently followed by 
tetanus, in addition to the regular surgical treatment. For 
