SOME EXPERIMEN TAL RESEARCHES ON THE USE OF MALLEINE. ;iI 
critics of Mr. Leblanc ? I regret that I did not destroy him, as I 
would have done with the permission of the owner. Perhaps I 
would have found at the post-mortem another solution to this 
important problem. However, this question of the possible cur- 
ability of glanders by malleic effects is not so new what is 
missing is the evidence that caii only be obtained by observation 
anc the autopsies of reputed recovered animals carefully traced 
afterwards in their existence. 
4 - Malleine , being a peculiar preparation from the culture of 
tie Virus of glanders, like turberculine and pneumo-basilline, are 
obtained from the cultures-of the tuberculous and peripneumonic 
viruses, must have the same qualities , in whatever aspect it is 
found. And whether as brute malleine of Roux, it is syrupous 
in aspect, of a dark-brown color, or like that of the Bureau of 
animal industry, it is delivered in solution, and then appears 
more aquous and of a lighter dark coloration; or, again, that 
it be like the one of Mr. J. Trautweiler, veterinarian, of Newark, 
who gave me two samples of German malleine, both of a very 
pale citrine color and almost like water (those are the three 
preparations that I experimented with), no matter what the 
physical character may be, the reactive effects must be the 
same. 
That the injection be made with I, 2 %, or 5 cubic centimeters, 
according to the indications of those who have prepared it, no 
matter ; the reaction will always appear to tell the story, and, if 
the effects are absent, when the malleine has been used in a case 
of glanders, is it not because the malleine has lost its qualities ? 
Therefore, the quality of the malleine is of a certain importance 
and its age must not be neglected no more than its physical’ 
qualities. 
Let us see. In August, 1892, I received from the laboratory 
of Dr. Roux, some brute malleine, and began to use it. I had 
been informed then that probably after two months it would have 
lost its properties. Observations 1, 2, 3, 4, 5, 6, 7, 8, 14, were 
the results obtained with it—used in solution, 2 V 2 c.c. at a dose. 
Observation 14 is made with it in April, 1893—that is eight 
