SOME EXPERIMENTAL RESEARCHES ON THE USE OF MALLEINE. 
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50. It being atmitted that malleine in cases of glanders 
gives rise to an elevation of temperature, an interesting and 
even important question is to know after what lapse of time , at 
what hour does this elevation manifest itself ? It is a question 
important to the practical point of view. As it is necessary to 
the observation to properly register the temperature at a certain 
hour, is there a time, a given moment, when the changes in the 
temperature will begin to be manifested. 
In the Clinica Veterinaria of June, 1894, appeared an article 
showing that this has been the subject of numerous observa¬ 
tions. Allow me to add some data obtained from my personal 
observations. They are resumed as follows : In one case the 
rise in temperature was noticed on the 4th hour after the injec¬ 
tion of malleine, in four after the 10th, in one after the 11th, in 
five after the 12th, in one after the 13th, in nine after the 14th 
—in one single case it only began towards the 24th, and reached 
its maximum towards the 35th hour. 
All these observations give an average of the time of the 
highest temperature between the 10th and the 14th hour, begin¬ 
ning before the 10th and extending to the 14th. 
From these it seems to me that the regulation of the tem¬ 
perature is much simplified. The injection being made in the 
evening, say between 10 and 11 o’clock, the first temperature 
being registered in the following morning, say from 7 to 8 A. M., 
will allow us to follow the rising of the thermometer every two 
hours until the expiration of the 16th hour, say until 2 o’clock 
in the afternoon, which is generally the beginning of the drop¬ 
ping of the temperature. 
In conclusion, allow me to call your attention upon a peculiar 
point presented by observation 29. This horse, certainly gland¬ 
erous by a first injection of malleine given on the 12th of April, 
received on May the 17th 2 cubic centimeters of Roux tuber- 
culine, and twelve hours later gave a reaction of more tlian 4 
degrees, with manifestations of local reaction such as malleine 
had never given before. 
Nocard tells us that “Tuberculine remains without effect on 
