346 
C. M. DAY. 
withers had been affected one year before and had been operated 
on at two previous occasions; first, on the left side at a time 
unknown, and on the right side about the 1st of July, when it 
discharged serum. The wounds from both operations, however, 
had entirely healed, and withers again presented their normal 
appearance until about one week previous to making cultures 
when they commenced to swell again. After washing the sur¬ 
face with a solution of chloral and soda a sterilized trocar was 
inserted about twelve inches posterior to the previous opening 
on right side, and a considerable amount of serum drawn off. 
Some of the serum was collected in a Sternberg glass bulb and 
in sterilized test tubes plugged with cotton, and saved for future 
use in the laboratory. Stab cultures were also made on agar- 
agar, gelatin, blood serum and bouillon directly from seium 
escaping from trocar. After seven or eight days the tubes were 
examined but no growths appeared. The conclusions are the 
case was a serous cyst perfectly free from micro-organisms of 
any kind. 
September i, 1893.—Case No. 2 was a sorrel pony, eight 
years old, without history of previous violence or injury being 
inflicted. The pony first showed lameness in left fore-limb 
about the 1st of June, 1893. After a short time a swelling 
appeared on left side of withers, which grew to be quite large, ■ 
very tender and sensitive to the touch. On August 29, 1893, 
the abscess opened without surgical interference and discharged 
considerable yellow, thick, creamy pus. Cultures were made on 
September 1, 1893, on gelatin, agar-agar and blood serum. A 
very vigorous growth appeared on the agar-agar and gelatin 
tubes, blood serum being sterile. Examination with a micro¬ 
scope showed the growth to be pu**^ culture of bacillus pyogeneus 
fcetidus. 
September 29,1893. Experiment No. 1.—Inoculated a mouse 
at root of tail; mouse died in twenty-four hours. An autopsy 
was held, but no pus was formed at seat of inoculation. Cul¬ 
tures were made from liver and heart blood, and some germ 
obtained that was used for the inoculation. 
