A BACTERIOLOGICAL STUDY OF EQUINE FISTUL.E. 
347 
October 4, 1893. Experiment No. 3.—Injected 2 drachms 
of bouillon cultures, thirty-six days old, into the muscles on the 
neck, just anterior to the cervical angle of the scapular, of a 
horse bought for dissection. October 19th considerable swell¬ 
ing around point of inoculation, very tender and sensitive to 
the touch; swelling remained large and sensitive until October 
25th, when the animal was destroyed and the parts dissected 
out. I found a cavity filled with a light-colored serous substance 
which, on examination with microscope, was found to contain 
pus corpuscles; it was evident that some of the tissue had 
broken down and a true abscess had formed. Cultures were 
made directly from the newly-formed abscess on agar-agar and 
potato; the growth which resulted was same as original. 
September 2, 1893.—Case No. 3 was a horse eight years old 
which had Poll-evil. The abscess had been lanced six days 
previous to making cultures, and was discharging a thick, yel¬ 
low, creamy-like, odorless pus. The history of the case could 
not be obtained. Cultures were made on agar-agar, gelatin 
and blood serum. A vigorous growth appeared on agar-agar 
tubes. On examination with the microscope, found it was a 
bacillus, but failed to identify it further. 
November 10, 1893. Experiment No. 1.—A mouse was 
inoculated with some of the original cultures; an abscess formed 
m thirteen days about middle of tail; no pus at seat of inocula¬ 
tion. November 17th made cultures from abscess on agar-agar, 
gelatin, potato and bouillon, and had no trouble in obtaining 
the same characteristic growth as the original. 
September 2, 1893* Case No. 4 was a bay mare, six years 
old, with a fistula on right side of the withers. The early 
history of the case could not be obtained. The abscess had 
been lanced seven days previous to our observation, and was 
discharging a thick, yellow, creamy-like odorless pus. Stab 
cultures were made on gelatin, agar-agar and blood serum. 
After remaining several days in the laboratory at the room- 
temperature a vigorous growth appeared in the agar-agar tubes, 
the gelatin and blood serum tubes being sterile. November 
