34-8 
C. M. DAY. 
20, 1893? a mouse was inoculated with some of the growth on 
agar-agar; mouse died in about thirty-six hours; cultuies were 
made from heart, blood and liver, and same germ obtained as 
in original. 
September 2, 1893* Case No. 5 - Fistula in a ba^ colt 
three years old which had never worked; no history of previous 
injury of the parts. The abscess made its appearance about 
three months before inoculations were made, withers swelled a 
great deal; abscess broke .on right side, discharged pus and 
swelling went down on this side but remained large on the left 
side. After closely clipping the hair over the swelling on the 
left side, and washing surface with a solution of bichloride of 
mercury 1 to 500, the abscess was lanced and a small amount 
of serum and pus escaped. Some of this was collected in a 
Sternberg bulb, and saved for future use in the laboratory; 
cultures were made on agar-agar, blood serum and gelatin 
directly from abscess,* and Esmarch rolled tubes were made m 
the laboratory from contents of bulb, but no growth appeared 
in them. The stab cultures on agar-agar and on blood serum 
were also sterile, but a slight growth appeared in the gelatin 
tube, and some transfer cultures were made from it. On exam¬ 
ination of the germ with a microscope, and after growing it on 
several media, it was found to be the staphylococcus pyogeneus 
citrius. The germ was pathogenic as the following experiment 
shows. October 26, 1893, inoculated a mouse at root of tail 
with growth on potato; mouse died in about eighteen hours; no 
pus formed at the seat of inoculation. Post-mortem was held, 
and stab cultures made from liver and heart blood which 
developed same organism. 
Case No. 6 was a colt three years old, which had never been 
worked. Presented a considerable swelling on both sides of 
withers without any history of injury. The swelling on the left 
side had been blistered with a blistering ointment. After clip¬ 
ping the hair and washing the surface With a solution of corrosive 
sublimate, about 1 to 500, the swelling on the left side was 
lanced. From the pus and blood which escaped stab cultures 
