A BACTERIOLOGICAL STUDY OF EQUINE FISTULA. 
were made on agar-agar, gelatin and blood serum. Some of 
the escaping pus and blood was collected in a Sternberg glass 
bulb and saved for making cultures in the laboratory. After 
remaining for six days in the laboratory at the room-tempera¬ 
ture, very vigorous growths appeared on agar-agar; on exam¬ 
ination this growth was found to consist of the sarcina lutea and 
the staphylococcus pyogeneus aureus. Experiments were made 
on a horse and several mice to determine their virulence, and 
both germs were found to be pathogenic as the following 
experiment will show. 
September 12, 1893. Experiment No. 1.—Injected 1 drachm 
of bouillon culture four days old of the sarcina lutea into the 
muscles of the neck of a horse, bought for dissection, a few 
inches anterior to the cervical angle of the scapula. The case 
was daily examined but no abscess was found, no signs of 
irritation. 
September 26, 1893. Experiment No. 2.—Injected about 
2 drachms of bouillon culture eighteen days old into the muscles 
of the neck of another horse, in about the same location as the 
previous case, but was again unable to produce an abscess. 
September 14, 1893. Experiment No. 3.—Mouse inoculated 
at root oi tail with some of the growth on agar-agar (sarcina 
lutea); mouse died in about twenty-four hours; cultures made 
from.liver and heart blood developed the same characteristic 
growth. No abscess was formed at seat of inoculation nor in 
any part of the body that could be detected on dissection. 
September 29, 1893. Experiment No. 4.—Mouse No. 2 was 
inoculated at root of tail with the staphylococcus pyogeneus 
citreus grown on agar-agar; mouse died in about twenty-four 
hours; autopsy was held but found no pus, and no abscess was 
formed at seat of inoculation. Made cultures from liver and 
heart blood on agar-agar, and obtained some characteristic 
growth as original. 
October 4, 1893. Experiment No. 5.—Growth from liver of 
mouse No. 2 was inoculated into mouse No. 3 at root of tail. 
The mouse did not die, but an abscess was formed at seat of 
