35 ° 
C. M. DAY. 
inoculation in about six weeks. On October 17, 1 S93, a large 
scab was lifted and cultures made from the pus and the same 
germ was obtained. 
September 13, 1893.—Case No. 7 was a gray mare, six years 
old, brought to college hospital for treatment, with fistula on 
neck just anterior to shoulder. The history of the animal 
showed she had been affected with strangles in the spring of 
1893, and several abscesses formed on the animal which broke 
and discharged pus, all of which healed except one on the 
neck, which remained open and continued to discharge pus 
through a fistulous opening. Cultures were made on gelatin, 
agar-agar and bouillon. After remaining for three days in the 
laboratory at room-temperature, vigorous growths appeared on 
all the tubes. On examination the staphylococcus pyogeneus 
aureus in pure culture was found in all the tubes. It was quite 
virulent as the following experiments will show. 
September 29, 1893. Experiment No. 1.—Inoculated mouse 
with growth from agar-agar tube; mouse died in about twenty- 
four hours. Autopsy was held and inoculations made from liver 
and heart blood, also cover glass preparations made from heart 
blood. Cultivations from heart blood produced same germ. In 
cover glass preparations the staphylococci could be seen with 
microscope. 
October 4, 1893. Experiment No. 2.—With the growth on 
agar-agar from heart blood of first mouse, a second mouse was 
inoculated which died at end of four days. Cultivations were 
made from liver and heart blood and same germ cultivated. 
No pus at seat of inoculation. 
October 6, 1893. Experiment No. 3.—Inoculated a dissect¬ 
ing case (horse) on the neck, just a few inches anterior to the 
cervical angle of the scapula, with about 6 drachms of bouillon 
culture twenty-four days old. On the following day, October 
7th, a considerable swelling appeared, very tender to the touch. 
October 8th, swelling much larger and more tender and sensitive. 
October 9th swelling about the same; tenderness remains. Oc¬ 
tober 10th swelling about the same, quite tender yet. October 
