THE DIAGNOSIS OF TUBERCULOSIS, ETC. 
with .ICC of tuberculin with as good results as when the same 
amount is used for four or five, the matter of expense alone is 
worth considering. It is well also to bear in mind that the 
arger the dose the greater the possibility that well animals will 
react. 
In June, 1892, when we first used tuberculin, we were uncer¬ 
tain about the dose proper, but finally decided to try .05CC and 
tested a herd of five mature cows, of rather more than average 
size, giving that dose. 
To our great surprise, where we had only suspected one, four 
reacted decidedly to the test. This unexpected result led us to 
believe that we might have used too large a dose. So, before 
killing these animals we tested another herd of twelve cows, 
dividing them into two lots of six each. One lot we injected 
with .05 cc tuberculin to a cow and the other lot with .25CC. 
Five of these twelve cows gave decided reaction and the diag¬ 
nosis m each case was verified by the autopsy. From the 
smaller dose the highest temperatures obtained were 105. 5, 
105. 1 in fourteen hours from the time of infection. From the 
larger dose 106. 7, 103. 8, 105. 5, in fourteen, sixteen and eigh¬ 
teen hours. This tiial tended to confirm our opinion that 05CC 
of tuberculin is at least sufficient to test a mature animal for 
tuberculosis ; and in subsequent tests we have not exceeded that 
amount, but have used from .05CC to .0150c according to the 
age and size of the animal tested. 
In all we have had thirty-one animals react under the test. 
Autopsies were made in each case and the diagnosis was uncer¬ 
tain in but two instances. Most of the animals were but slightly 
affected. Five or six of them would be considered advanced 
cases and were readily diagnosed by a physical examination- 
Two of the thirty-one were calves that were injected with .015CC 
of tuberculin each. The thirty-one animals together were in- 
jected with less than 1.40c of tuberculin. 
Now to make a general comparison of results with some of 
those who have used a much larger dose. 
We find that the average maxium temperature in these 
