12 
VETERINARIUS. 
the nasal secretion of a glanderecl horse and found “ the transpar¬ 
ent thallus of a mycelial fungus, and numerous masses of reddish- 
brown streptococci;” this observation was undoubtedly correct, 
but the organisms owe their origin to the food, and can be found 
in the nasal secretion of almost any horse, and, therefore, they can¬ 
not have anything to do with the cause of glanders. During the 
next twenty years no similar observations were recorded. Vir¬ 
chow, in his classical studies on the pathological anatomy of the 
neoplastic production in glanders, says that one must look upon 
the contagion as of an acrid or irritative nature, whether it worked 
from the blood or directly upon the tissues. In 1868, several 
new works appeared upon this question. 
Chauveau stated that the inficiens (or infecting material) of 
glanders was contained in the solid elements of the infecting or 
infectious material; in his work he gave an exhaustive description. 
He took 10 c-c-ms of pus from the pulmonary abscess of a 
glandered horse and diluted the same with 200 c-c-ms of water. 
This mixture was frequently stirred up and filtered so that nothing 
was left but the solid elements. He then mixed the latter with 
500 c-c-ms of water and allowed it to stand over night. The fluid 
was then gently removed with a syphon and the refuse at the bottom 
of the vessel again diluted with another 500 c-c-ms of water and 
allowed to stand another night. This water being removed as be¬ 
fore, still another 1,000 c-c-m’s of distilled water was added, the 
mass being again stirred up and then filtered, the solid element 
being then mixed with very little water. The inoculation of sev¬ 
eral horses and donkeys with this solid material was followed by 
the eruption of glanders in four days, while similar experiments 
made with the last filtrated water gave negative results. Chauveau 
drew the following conclusion from these experiments: “ That 
the leucocytes (or white blood-cells) suspended in the virulent 
fluids may be considered as the receptacles of the virus,” which 
later observers have confirmed. 
Hallier and Zurn conducted numerous investigations, but neg¬ 
lected to confirm their conclusions by inoculation experiments 
with horses. Semmer, of Dorpat, confirmed the conclusions of 
Zurn and Hallier, but his experiments gave negative results. 
