14 
VETERlNARlUS. 
was also the case in using Weigert’s gentian-violet picro-carmine 
method. Numerous other methods and many coloring materials 
were also tried, but with no satisfactory results. Methyl-blue was 
the only material that gave any hopes; it was used as follows: 30 
c-c-ms concentrated alcoholic solution of methyl-blue, 100 c-c-ms 
of kali eausticum, 1 to 10,000 of aqua. Place sections of tissue in 
the above solution for about five minutes, and then wash quickly 
in a solution of 1 per cent, acetic acid, then in absolute alcohol, 
then in cedar oil, and mount in Canada balsam, which should not 
be dissolved with chloroform, but with xyol or turpentine for all 
aniline colors. Careful microscropic examination of sections treated 
as above will show the presence of very delicate bacilli in the periph¬ 
eral portions of glanders noduli, which bear a very strong resem¬ 
blance to those of tuberculosis; they could not be putrefactive bac¬ 
teria, as the organs were hardened when perfectly fresh, and their 
presence was limited to the noduli and not scattered indiscriminately 
over the specimen. The small number present, and the fact that 
they were entirely wanting in some specimens, did away with any 
doubt regarding their genetrical connection with the pathological 
products. As the coloring method did not allow of any positive 
conclusion as to the value of these bacilli, the investigators had 
recourse to other methods. 
Cultivation is undoubtedly the most satisfactory method of 
demonstrating the presence of micro-organism in a tissue or organ. 
By this method Strauss and Chamberland in inoculated anthrax 
were able to demonstrate the presence of anthrax bacilli in the 
blood of foeti of pregnant guinea-pigs, in direct contradiction to 
the microscopic experiences of all previous observers. 
On the 14th of September, 1882, Dr. Loeffler commenced his 
experiments. In order to render them as certain as possible, he 
had recourse to a very large selection of cultivating media, such 
as sterilized and solidified blood serum drawn from the horse and 
sheep, infusions made from the flesh of horses, rabbits, hens, 
cattle and man, as well as various vegetable substances. 
To prevent the pieces of the organs used for cultivations be¬ 
coming polluted, they were . placed in a vessel containing a 5 per 
cent, solution of carbolic acid for flve minutes, and then put in a 
