GLANDERS. 
15 
1 per cent, solution of corrosive sublimate, then placed on a clean 
glass plate. The hands of the operator, as well as the instruments, 
were disinfected before use. Great value could be placed upon 
cultivations from the liver and spleen, as the accidental pollution of 
their interior was almost impossible under such circumstances. A 
section was made in the vicinity of a glanderous nodule, and then 
another down on the same with afresh knife, but not into it; 
the node was then seized with sterilized forceps and torn apart; 
the purulent material in such a growth thus escaped contact with 
any instrument, and was immediately inoculated upon the various 
media by means of sterilized platinum needles, a fresh one being 
used in each case. Seventy cultivations were made and placed in 
a thermostat having a temperature of 38° C. During the first 
two days no important changes occurred; on the third day they 
became clouded with the exception of that made from the juice of 
stewed prunes. The surface of the consolidated blood serum be¬ 
came covered with numerous transparent drops having a yellowish 
color, giving the surface of the serum the appearance of having- 
been sprinkled with some fluid ; the majority of the tubes con¬ 
tained nothing but these drops, but in a few some isolated non¬ 
transparent white or yellowish colonies appeared, easily distin¬ 
guished from the yellow drops, and gave the impression that they 
were of a pollutive nature or accidental occurrences. A micro¬ 
scopic examination of the yellow transparent colonies revealed the 
presence of very fine delicate bacilli corresponding in all essentials 
with those found in sections of the pathological neoplasms of 
glanders. The same bacilli were found on all the cultures except 
the prune juice infusion. An occasional tube was found polluted 
with a micro-organism of another form and character. Some 
tubes inoculated with material from diseased lymph glands had 
development of colonies. The bacilli varied very little in length, 
some being between one and two-thirds the diameter of a red 
blood corpuscle; they were straight or very slightly curved, being 
somewhat shorter and thicker than the bacillus of tuberculosis. 
The bacilli generally occurred in lateral pairs. When examined 
in some cultivating medium suspended in a hollow object-glass 
they exhibited a very active Brownian movement, but idiosyn- 
