HOG CHOLERA. 
27 
December 29 No. 41 died from the effects of the bacteria only, 
that is of septicaemia, or blood poison, as can be seen from au¬ 
topsy No. 1. Its blood and the tissues of the spleen were liter¬ 
ally swarming with the well-known bacteria. Though frozen solid, 
cultivations grew from the spleen and two rats inoculated with 
1 c. c. m. of a boullion cultivation from the same succumbed in 
forty-eight hours. Pure cultivations were obtained from the rat, 
and have since been retested and again proved fatal. 
Nos. 44, 42 and 46, that had been vaccinated twice in thirty 
days, were somewhat off their feed and lame a few days, but 
entirely recovered, while 41, that was only inoculated once, suc¬ 
cumbed. 
On December 12 numbers 45 and 47 were again inoculated 
with six fluid grammes of a boullion culture that had previously 
been tested upon rats and proved fatal. These pigs also recov¬ 
ered completely, and may be considered proof against swine 
plague. 
We have therefore positively proved that we can prevent 
swine plague by inoculation of healthy swine with an artificially 
prepared virus. 
We have now hogs 44,42 and 46, that will to a dead certainty 
withstand all exposure to infection, if placed among sick hogs, as 
they were tested with a vaccine of unusual malignancy for this 
year’s swine plague in the State. 
The fact that No. 41 succumbed so quickly to the same virus, 
though the liveliest pig in the lot, shows that one vaccination is 
not sufficient. 
Nos. 45 and 47 will probably also withstand any natural test, 
as they have been inoculated three times. 
No. 43 has been purposely left without the final test inocula¬ 
tion, but will, I think, stand it also. While there is an immense 
amount of detail work yet to be done, we have got to fix the 
exact amount of inoculation necessary, and the time which is 
necessary to elapse for the constitutionalization of the virus so 
that immunity can be produced. I hope to find some small 
animal so susceptible to the virus, that by passing it through a 
short series of such animals, it will attain a constancy of virulence 
