GLANDERS. 
73 
GLANDERS. 
By Veterinarius. 
A Contribution for the prize offered by the TJ. S. Veterinary Medical Association 
for Papers published in the American Veterinary Review. 
(Continued from page 16 .) 
Dr. Loeffler’s next experiment was to develop cultures from 
the greatest number of glandered horses possible, and see if the 
same micro-organism would invariably develop. The first horse 
examined presented, besides other symptoms, an exquisite case of 
chronic glanders in the spleen and liver. Cultivations were made 
from the liver and spleen. Those from the liver did not yield 
many colonies, but what it did were very characteristic; the 
splenic cultivations remained sterile. Bacilli could not be demon¬ 
strated in the tissues by any method of colonizing. Experiments 
and examination of the organs of another horse gave more satis¬ 
factory results, the characteristic bacilli being easily found when 
subjected to the methyl blue process as already mentioned. Cul¬ 
tivations gave the previously described yellow vesicles. The third 
horse presented the phenomena of acute glanders in optima forma 
both in the septum nasi and lungs. In cultivations from the 
lung nodule in blood serum the development was so profuse that 
the surface appeared as if covered with a yellow transparent coat¬ 
ing ; the single vesicles could only be distinguished at the edges. 
The characteristic bacilli were easily demonstrated in sections of 
the ulcers and pulmonary noduli. A fourth horse, which was 
very old, presented chronic ulcerations with indurated edges in 
the septum nasi, the cartilages being necrotic underneath. Tough 
fibrous noduli, many of which were calcified, were found in the 
lungs; the liver was also the seat of large noduli, the centre of 
which had undergone puriform softening. In this case cultiva¬ 
tions were made from nodules from the liver as well as the lung, 
in order to guard against contamination from without. In this 
case the yellow vesicles again developed on blood serum, but in 
small colonies, as in the first case of chronic glanders, a result 
that was anticipated from the age of the neoplasms. 
