GLANDERS. 
75 
greenish-white fluid oozed out. The inguinal glands of the left 
side were also swollen and full of yellow centres. Spleen en¬ 
larged ; liver in a similar condition; both filled with greenish 
noduli. Kidneys intact. Lungs contained greyish-red neoplasms. 
Testicles both hypertrophied, the inner and outer membranes be¬ 
ing adherent. Left foot swollen, and on cutting through the skin 
a purulent material escaped. Left side of the septum nasi ulcer¬ 
ated and covered with crusts. The presence of bacilli in the nu¬ 
merous tissues left no doubt as to the nature of the disease. 
The third case gave the same results. 
These cases were selected from a great number of animals 
that were subjected to experimental inoculation, and show that 
guinea-pigs inoculated with glanders material develop a very char¬ 
acteristic disease, and that the same specific micrc-organisms were 
produced as occur in equine glanders, as demonstrated by micro¬ 
scopic examination and cultivation experiments. 
Dr. Loeffler’s next experiment was to show that the bacilli 
thus derived and cultivated from glanders noduli and products 
w T ere not accidental occurrences in the disease, but the actual etio¬ 
logical moment, and that the disease itself could also be generated 
by inoculations from pure cultivations of the micro-organisms. 
Eight days after a cultivation of glanders material had been sown 
upon blood serum, a small quantity was taken up upon a previous¬ 
ly heated platinum wire and reinoculated upon fresh material. 
Eight days later another cultivation was again made in the same 
manner, and twelve days later another. It was assumed that 
none of the original organisms were present in the fourth genera¬ 
tion. The cultures consisted of nothing but the previously con¬ 
sidered fine bacilli. Thirty-six days from the time the first culti¬ 
vation was sown some of the material from the last cultivation 
was mixed with a small quantity of sterilized blood serum and in¬ 
oculated into the Schneiderian membrane and both shoulders of a 
horse apparently free from any symptoms of glanders. The 
syringe used for the experiment was sterilized by exposure to 
160 deg. C. The animal’s temperature began to rise within 
forty-eight hours, measuring 39.5 c\eg. 0. in the rectum. One 
could feel a small elevation at the locus inoculationis. An ulcer 
