OLANDERS. 
77 
to the animal being already afflicted with glanders, a renewal of 
the experiment was necessary. 
The subjects were a healthy horse, 20 years old, and one 2 
years old. The old horse was inoculated with virus from a pure 
cultivation of the original material in the tenth extra organismal 
generation. The young horse was inoculated with a culture of 
the fifth generation, that was made from the testicle of a guinea- 
pig that died 24 days after being inoculated, and which had been 
inoculated with the fourth generation of the original material. In 
order that the inoculation of these horses should be as severe as 
possible, the material was introduced in considerable quantities in 
both shoulders, the breast, flank, and the back of the nose. 
Nothing was injected into the mucosa of the nose, in order to see 
if secondary eruptions would develop in the same. 
In a few days, at the locus inoculationis, a diffuse oedematous 
tumefaction was to be seen. The animals ate poorly, were stiff 
in their movements, and had staring coats. In the course of eight 
days the tumefactions opened and secreted a cloudy greenish-yel¬ 
low material. On the 12th day an ulcer appeared on the fore¬ 
head of the younger horse, about the size of a quarter-dollar, pen¬ 
etrating to the bone, with irregular indurated edges. In both 
animals a nasal secretion appeared, which desiccated in thin, dry 
crusts upon the wings of the nostril, and, finally, development of 
ulcerations in the mucosa of the nasal cavities, which placed the 
glanderous nature of the iuduced disease beyond-all question. 
Both animals became daily more and more emaciated. The 
elder died first. 
The following is the result of the autopsy: At all the points 
of inoculation, ulcerations the size of a quarter of a dollar and 
larger, were present; the same were covered with thick crusts of 
dessicated secretions and hairs; they secreted a yellowisli-white 
material. The soft tissues beneath these ulcerations were degen¬ 
erated, and almost in a fluid condition ; the surrounding subcutis 
was infiltrated with a purulent material. The ulcerations in the 
neck were in connection with lymphatics that formed cords ex¬ 
tending to the axillary lymph glands, which were as large as a 
hen’s egg, their cut surface presenting numerous small yellow 
