GLANDERS. 
79 
tissue of the sheath was of a gelatinous character, and the lymph 
glands in and around it were hypertrophied. Extensive cavities 
were observed in a number of muscles, filled with a clouded fluid 
of a yellowish-white color. The nasal mucosa were filled with 
numerous noduli and ulcerations, the former being gray or yellow¬ 
ish in color. The surrounding mucosa was liypersemic and red ; 
the edges of the turbinated bones were complicated with these 
productions; no ulcerations were present in the mucosa of the 
pharynx, larynx, trachea and bronchial tubes. The submaxillary 
glands were as large as a walnut and exceedingly indurated, but 
not attached, and on cutting them numerous yellowisli-white neo¬ 
plasms were observed. In the lungs six small noduli were found, 
surrounded by a somewhat dense parenchyma and liypersemic tis¬ 
sues. The adjoining lymphatics were enlarged. Spleen hyper¬ 
trophied, soft, and of a dark-brown color. The liver, kidneys, 
heart and muscles presented nothing abnormal beyond a slight 
cloudy swelling. 
The clinical as well as the microscopical phenomena permitted 
of no doubt as to the nature of the induced disease. The charac¬ 
teristic bacilli were present in the tissues, and cultivations from 
both of the above animals. 
To test the question further, a number of guinea-pigs were 
inoculated with fresh material as it was taken from the horses. 
One of them, inoculated with material from the old horse, died 
in 15 days. The autopsy and microscopic examination of the 
neoplastic products gave full evidence of the nature of the dis¬ 
ease. Similar results followed in each of the animals inoculated. 
These investigations led to the following results: That in the 
specific products of a typical case of glanders in the horse, and the 
neoplasmata produced in guinea-pigs inoculated with material 
from such a horse, a bacillus was found which can be demonstrated 
by traction, and especially by cultivation upon blood serum. 
Pure cultivations of many generations caused glanders in horses 
and guinea-pigs upon inoculation, which is sufficient proof that 
those bacilli are the etiological moment in glanders. 
Shortly after the publication of the above experiments, Bon- 
chard, Capitan and Charrin published results of similar studies. 
