108 
VETERINARIUS. 
and liver. The noduli had exactly the same microscopical appear¬ 
ance as the lymphomatoid neoplasmata described by Virchow and 
Klebs in ileotyphns. Bacilli were present in the centre of these 
objects in great numbers, frequently in the body of lymphoid 
cells, and in the cut section of blood vessels between the red blood 
cells. Cultures upon blood serum gave the characteristic yellow 
vesicles. It will thus be seen that the field mouse affords all the 
conditions that are necessary to test the infectious elements in 
glanders, and is especially valuable for inoculation in doubtful 
cases in the horse. This mouse is not affected by the bacteria of 
mouse septicaemia in contradistinction to the white mouse, while 
the latter is nonsusceptible to the action of glanders bacilli. 
The Biological Peculiarities of the Bacilli in Different 
Cultivating Media. 
As already mentioned glanders bacilli develop exceedingly 
well upon the stiffened blood serum from horses or sheep. 
On the third day after being sown the bacilli appear upon the 
surface of the serum in transparent yellow vesicular colonies. On 
penetrating one of these drops gently with a platinum needle, and 
withdrawing same gently it will be seen to be connected with the 
colony by a very delicate thread of its substance, which shows 
that it possesses a tough mucoid, viscid character. The appear¬ 
ance of such a colony changes after it has been from eight to ten 
days in the thermostat. They lose their yellow transparency and 
become milk-white; this is especially marked when the cultiva¬ 
tions were made upon greenish colored equine blood serum. A 
microscopic examination of these colonies revealed the presence of 
numerous rod-like organisms possessing a high degree of refraction, 
which at first gave the impression of large free spores, but proved 
to be quite otherwise, from their angular form and irregular size; 
they are undoubtedly small crystals, the precipitation of which 
causes the whitish change of color in the vesicles; the nature of 
these crystals was not determined. 
The bacilli also develop in a similar but not so luxuriant 
manner in the stiffened blood serum of cattle. They also develop 
well in infusions made from the flesh of man, horses, sheep, 
