110 
VETERINARIU8. 
if you treat cultures of the glanders bacilli in a like manner. 
The microscopic examination of pus-bacilli shows them to be 
larger than those of glanders, and exquisitely motile. The obser¬ 
vations of Kitt and Weichselbaum confirmed those of Dr. Loeff- 
ler, without their having any previous knowledge of his experi¬ 
ments. These experiments were proved upon guinea-pigs and 
field mice with the most satisfactory results. Dr. Loeffier kept 
one cultivation upon a potato medium from the 1st of April, 1883, 
to the beginning of July, 1884 ; it was found to have lost much 
of its virulence by experimental inoculation, as great quantities 
only caused slight local reaction in guinea-pigs. By means of 
these media the limits of temperature under which these bacilli 
thrive was determined. 
In a meat-water peptone solution, with or without agar-agar, a 
morbid development of these bacilli could be distinguished at the 
ordinary temperature. The cultures in flesh-water peptone gela¬ 
tine remained sterile under the same circumstances—that is, at a 
temperature of 15° to 20° C. At over 20° C., and at a degree 
which did not cause the gelatine to become fluid, but still af¬ 
fected its consistency, the bacilli developed in the shape of a pro¬ 
liferate ; at 22° C. a spur of development was observed; at 25° 
C. the potato had a delicate yellow coating; at the end of five 
days, while at 37° C., a profuse development occurred within three 
days ; at 27° C. the growth was not nearly so luxuriant; between 
30° and 40° C. they seem to proliferate with a like rapidity and 
profusion; 41.5° C. somewhat checked the development; the 
color was already of an amber shade, and the individual colonies 
remained very small. The microscopic examination showed the 
bacilli to be very short, and to have a stunted appearance. At 
43° C. they remained about the same, while all proliferation 
ceased when the temperature was raised to 45° C. The lowest 
temperature limit in which glanders bacilli retain their biological 
activity is a matter of the greatest importance. A temperature 
of 25° C. is no unfrequent occurrence for many days at a time 
during the summer months. It is possible that the fluids within 
such stables may provide suitable media for these bacteria to re¬ 
tain their vitality outside of the animal organism. It is well 
