GLANDERS. 
113 
fuchsin is used, the section must remain in it from fifteen minutes 
to half an hour. A solution consisting of 10 c-cms. of aqua dis- 
tilli., to which has been added two drops of concentrated sul¬ 
phuric acid and a few drops of five per cent, oxalic acid solution 
for the treatment of sections colored in methyl-blue, is much bet¬ 
ter than acetic acid trapacolin. This solution removes the color 
from the tissues and nuclei without having any effect on the 
bacilli. The duration of the action of this decolorizing agent can¬ 
not be exactly given, being a matter of practical experience, de¬ 
pending on the thickness of the sections of the tissues. Thin sec¬ 
tions of pulmonary tissues should remain in the alkaline methylen- 
blue solution for two to five minutes, and then wash for five min¬ 
utes in the oxalic acid mixture, then with absolute alcohol, cedar 
oil, and mount. It is an advantage to place the sections in a so¬ 
lution of caustic potash, 1 to 10,000 of aqua, for a few minutes 
before immersion in the coloring fluid. If the coloring has been 
successful, the bacilli will be very easily defined by their dark 
blue border. I found it better when using methylen-blue and an 
alkali, to allow the sections to remain in the coloring fluid for at 
least twenty-four hours ; then transfer to the oxalic acid mixture, 
then into 60 per cent, alcohol, and then into a contrast stain eosin; 
from there into 90 per cent, alcohol, then into absolute alcohol 
and oil of cedar, and mount in Canada balsam. This method I 
found by far the most satisfactory. In old noduli the colonizing 
test is not to be depended on, the cultivation method being the 
most reliable. Glanders bacilli are thoroughly decolored by treat¬ 
ing them with permanganate of potash in the same manner as Lust- 
gar ten claims peculiar to syphilis. By treating a fresh glanders 
nodule, according to Lustgarten’s method, with permanganate of 
potash and submitting the same to microscopic examination, you 
will find a circumscribed cellular phenomena, in which the limits 
of the alveoli are filled with cells which are of an epitheloid char¬ 
acter, as well as small round cells which also fill the interstitial 
framework. The capillaries surrounding the nodulus will be 
found distended with blood-cells, with an occasional diapedefis. 
In the center of the nodule bacilli can be seen collected in small 
groups, and closer examination demonstrates the presence of an 
