GLANDERS. 
159 
microscopical examination. The following is the course of these 
experiments : 
Two field mice, inoculated with freshly-dried material, died on the 4th day, 
of typical glanders. 
Two field mice, inoculated with the same material, after 4 days’ desiccation, 
died—the first on the 3d day, of glanders; the second on the 6th day, of 
glanders. 
Two field mice, inoculated with material dried for seven days, died—the 
first on the 3d day, of glanders; the second on the 4th day, of glanders. 
After 10 days’ drying, two more inoculated. The first died on the 4th day, 
of glanders; the second on the 5th day, of glanders. 
After 13 days’ drying, two were inoculated. The first died on the 6th day, 
of glanders; the second on the 7th day, of glanders. 
After 17 days’ drying, two were inoculated. The first died on the 4th day, 
of glanders; the second on the 6th day, not of glanders. 
After 20 days’ drying: The first died on the 2d day, not of glanders; the 
second on the 6th day, of glanders. 
After 28 days’ drying: The first died on the 3d day, of glanders; the se«- 
ond on the 4th day, of glanders. 
After 32 days’ drying: The first died on the 3d day, of glanders ; the sec¬ 
ond on the 4th day, of glanders. 
After 40 days’ drying : The first died on the 4th day, of glanders ; the sec¬ 
ond on the 5th day, of glanders. 
After 60 days: Both died on the 4th day, of glanders. 
After 89 days: One inoculated died on the 5th day, of glanders. 
After 715 days: Four threads sown on potatoes; no development. 
These experiments demonstrated, beyond doubt, that glanders 
bacilli that have been dried for three months may retain their 
biological activities. 
It is not necessary to assume that permanent spores are formed 
by these bacteria, as others which do not form spores have been 
known to retain their activity for the same period under a similar 
course of treatment. When it is taken into consideration that the 
nasal secretion of glandered horses contains micro-organisms of 
various kinds, and that fermentive processes almost always occur, 
it can be understood why numerous experiments made with this 
material have given negative results. If, as has been shown, the 
dried bacilli de not retain their activity for over three months, it 
is still possible that they may retain action longer in the warm, 
moist air of stables. 
This supposition can also be tested by experiment, by observ¬ 
ing their conditions on different media after the lapse of varying 
