160 
VETERINARIUS 
periods. A great number of cultivations were further inoculated 
after having been in the thermostat for from one to four months, 
and old cultures all proved to have died out. Those one hundred 
days old developed sparely, as well as those of ninety, eighty, sev¬ 
enty, sixty, fifty and forty days. Cultivations of four weeks de¬ 
veloped in tolerable quantities, but unevenly upon blood serum 
and potatoes isolated colonies were generally observed. Cultures 
of from fourteen days to three weeks showed normal properties. 
It can therefore be seen that these bacteria do not retain their 
activity long, even when placed under favorable conditions to 
their continued development. 
Action of Disinfecting Agents upon Glanders Bacilli. 
Heat is among the best of these; its destructive action on the 
infectious material from glandered horses has been well known 
for a long time. 
Bourgelat subjected purulent glanders material to distillation; 
product and refuse both inactive. 
Abildgaard and Yiborg found the same material inactive after 
being heated to 45° C. 
Benault cooked it with the same result. Dr. Loeffier’s experi¬ 
ments upon pure cultures of glanders bacilli were followed by the 
same experience; all cultures, whether the bacilli demonstrated 
an uncolored sporoid centre or not, proved inactive after being 
cooked once. 
A potato cultivation which had developed at a temperature of 
38° C. for a period of seven days, was rubbed up with a certain 
quantity of freshly distilled water until it became quite apales- 
cent; 5 ccms. of this material was then placed in a number of 
sterilized test tubes, when each was placed in a water bath, at 
50°, 60°, and 70 u C., for ten minutes. On being taken out, some 
sterilized potatoes were immediately inoculated from them, while 
others were sown from the non-heated sterilized mixtures in other 
tubes. After the lapse of three days, the potatoes which had 
been sown,from the non-heated tubes and from that heated to 50° 
C., were covered with an amber-colored mass, while the others 
remained free from any development. This experiment was re- 
