GLANDERS. 
161 
peated with material prepared in the same manner that had been 
heated tc 52° and 55° C. At the latter temperature the bac¬ 
teria were found to have been killed, while at the former they re¬ 
tained more or less activity. 
The subjection of glanders bacilli to a temperature of 55° C. 
is sufficient to kill them. This is the same temperature that kills 
anthrax bacilli which are free from spores. 
Chlorine gas and carbolic acid have been recommended as the 
most trustworthy germicides. 
Gerlach mixed four grammes of the nasal secretion from a 
glandered horse with fresh chlorine water, and after two hours 
inoculated a healthy horse with the same. Result negative. A 
piece of the septum nasi, covered with ulcerations, was placed by 
the same authority in a solution of carbolic acid, 1 to 24 parts of 
water, for 30 hours; inoculation negative. He also placed freshly 
cultivated nasal secretion in the same mixture for only a few min¬ 
utes, and on inoculation the result was negative. 
Reynal produced similar results with chlorine. Peach and 
others give similar testimony. 
Dr. Loeffler experimented with carbolic acid, corrosive subli¬ 
mate, chlorine water, and permanganate of potash. The material 
used was four-days-old cultures of the bacilli upon potatoes 
which were rubbed up with a solution of meat-infusion-peptone. 
Sterilized silk threads were impregnated with this fluid, and 
soon as they were dry and ready for experiment. The disinfect¬ 
ing fluid was placed in sterilized crystalization vessels to the 
amount of 20 to 30 ccms. 
Experiment No. 1.—A number of such threads were placed 
in a 2 per cent, carbolic acid solution, and the same number in a 
5 per cent solution. After the lapse of 2, 5, 10, 20, 30 and 40 
minutes, they were removed and washed in distilled water and 
placed upon potatoes ; the same being done with saturated threads 
that had not been so treated. After six days the last were seen 
to be surrounded by a yellowish-brownish covering, as were also 
those which had been subjected to the action of the 2 per cent, 
disinfectant. The development of those placed for 2 minutes in 
the 5 per cent, solution was not so prolific as was the case with 
