INOCULATION AGAINST ANTHRAX. 
311 
Not being satisfied with this trial of M. Arloing’s dried pow¬ 
der, a fresh quantity was obtained from Paris, and on the 10th 
of January a heifer and steer, about ten months old, were inoc¬ 
ulated at the veterinary college, the operation being perfected by 
a second operation after the prescribed interval. 
The testing of these fresh vaccinated cattle was rendered 
possible by our having to investigate an outbreak of quarter-ill 
among the young stock of Mr. Godman, Horsham, who, having 
lost one animal, was desirous of having his remaining ones pre¬ 
ventively inoculated. The case which had proved fatal being 
considered a typical one material was taken from it with which 
to test our vaccinated animals at the college. Both the cattle 
and one guinea-pig, unprotected, had injected into their con¬ 
nective tissues a little of the muscle-juice obtained from the dead 
ox. In all these animals this inoculation proved fatal. The 
guinea-pig died in twenty-four and a half hours, the steer in forty- 
four and a half hours, and the heifer in forty-seven hours. After 
death, examination disclosed well-marked lesions of quarter-ill. 
Being now rather dissatisfied with our trials of the dried mus¬ 
cle-juice as a protective “ vaccine,” attention was turned to the 
second method already referred to for producing immunity, viz., 
that of intra-venous injection of simple diluted virus. Experi¬ 
mentation on this system was rendered possible by the generous 
offer of Major Algernon Percy, of Hodnet Hall, Shropshire, to 
place certain animals at our disposal for this purpose. The 
opportunity to commence this trial was afforded on November 
11th, when, in response to a telegram from Major Percy, Prof. 
Penberthy proceeded to Hodnet. On the morning of the 12th, 
in the presence of many interested in the experiment, four calves, 
duly marked for identification, had a regulated quantity of mus¬ 
cle-juice, previously mixed with water, injected into the jugular 
vein. This muscle-juice was prepared by straining through 
coarse linen, and was conveyed into the blood stream by means 
of a hypodermic syringe. Following the operation, the four 
calves, with a similar number of uninoculated, also marked for 
identification, were turned into a pasture and treated in every 
way alike, except that the temperatures of the inoculated were 
