P. PAQUIN. 
356 
directed researches and experiments, in these days of scien¬ 
tific progress, are often sufficient to elucidate the most ob¬ 
scure point or question. Black leg, however, has been much 
studied in Europe. Indeed the veterinarians already men¬ 
tioned (of the Veterinary School of Lyons) have long and 
patiently experimented (to the great good of the whole 
world). They have shown beyond all doubt, that it is speci¬ 
fic and transmissible. Their inoculations from cattle to cattle, 
cattle to sheep, and to rabbits are conclusive. And yet, with 
all that, most of people on this continent have doubted to this 
day. Perhaps we are to blame for that, for our stockmen 
and farmers have not been informed wide ly, as was done in 
Europe, at government expense. 
In order to lay the matter clearly before the masses, we 
made a few experiments. First, we too inoculated cattle 
from cattle, then sheep from cattle, rabbits from cattle and 
rabbits from rabbits, and though some writers have denied 
until now the transmissibility of the disease by inoculation, 
we have repeatedly succeeded in accomplishing it with seri¬ 
ous or deadly results. 
My first inoculations were made early in 1887, when fil¬ 
tered, fresh and unmodified fluid of a crushed piece of flesh 
from a black leg tumor was inoculated to four rabbits with 
fatal effects in two, and in one heifer with the effect of caus¬ 
ing a characteristic tumor and lameness. 
In examining the lesions of each sick inoculated animal, 
they presented the specific appearances of black leg, and the 
microscope revealed the true germs that produce it. 
These limited experiments were certainly in line with the 
published reports of the French investigators. 
Later the same year, 1 caused the practice of inoculation 
of unmitigated fluid from a liver, with the production of the 
characteristic tumor with fatal results in two calves five and 
seven months old respectively. This only confirmed former 
conclusions. 
These spasmodic experiments could not be made of imme¬ 
diate practical use. Without assistance I had to travel offi¬ 
cially almost constantly, and methodical laboratory work was 
almost entirely out of the question. 
Late in 1889, however, we had occasion to test again the 
transmission of black leg and protective inoculation with a 
modified virus. 
The following short table gives the results of some of our 
latest inoculations practiced with very strong virus taken 
fresh from typical tumors, or from livers or lymph glands of 
diseased subjects. 
