/ 
522 E. A. y. SCHWEINITZ. 
ply to these substances. We shall, therefore, refer to the 
ptomaines and albumin by the names given above. 
The first of our experiments that we will record were 
made with sucholotoxin. 
Experiment I.—Two guinea-pigs, each weighing about 
three-fourths of a pound, were treated with a solution of 
about 0.05 gramme of sucholotoxin each. The solution was 
introduced under the skin of the inner side of the left thigh. 
Immediately after the operation the animal appeared uncom¬ 
fortable, but was not made ill. For a few days there was a 
rise in temperature, and also a slight swelling at the point of 
inoculation, which, however, disappeared in about five days, 
and the animal was then well. 
Two more guinea-pigs were now selected as checks, ap¬ 
proximately of the same size and weight as those which had 
been treated, and the four animals were then inoculated with 
0.1 c. c. of hog cholera virus each (o. 1 c. c. beef-infusion and 
peptone culture one day old, plus 0.2 of sterile, normal salt 
solution). This is the dose which previous experiments made 
in the Bureau had shown to be the proper quantity to kill a 
guinea pig in from eight to ten days. The inoculations with 
the virus were also made subcutaneously in the thigh. The 
checks died in eight and nine days. Post-mortem examina¬ 
tion showed a large swelling at the point of inoculation, infil¬ 
tration of a purulent, grayish substance into the connective 
tissue, and necrosis of the superficial layer of the muscles of 
the thigh ; enlargement and reddening of inguinal glands ; 
Peyer’s patches enlarged and pigmented; liver pale, and cov¬ 
ered with a number of necrotic foci; spleen very much en¬ 
larged, dark-colored, and friable. Cover-glass preparations 
from the spleen and liver showed hog cholera germs. This 
was the characteristic appearance of all the check guinea-pigs 
upon post-mortem examination, and it will not be necessary 
to repeat these details. 
Of the animals which had been first treated with the sub¬ 
stance mentioned, and afterward inoculated, one died two 
days after the last check. Autopsy revealed the following: 
At the point of inoculation in the left thigh the subcutaneous 
