524 
E. A. V. SCHWELN1TZ. 
made from the spleen showed hog cholera germs. Beneath 
the peritoneum in the region of the spinal column, and in the 
mesentery was a considerable number of small grayish tuber¬ 
cles. Several other experiments were made by treating 
guinea-pigs with the albumin in varying quantities, all show¬ 
ing resistance, and subsequently immunity. 
Experiment III.—Three guinea-pigs were treated with 
sucholo-albumin, o.i gramme being given to each, subcuta¬ 
neously in the thigh. The albumin for two of the animals 
was derived from cultures containing blood-serum ; the albu- 
mose given to the third was from ordinary beef-infusion pep¬ 
tone culture. Ugly ulcers formed at the point of inoculation, 
which healed, however, in from ten to fourteen days, and the 
animals, with the exception of a slight rise of temperature, 
were well. 
Two checks were again selected, and the five animals were 
inoculated with o. i c. c. hog cholera virus. The checks died 
respectively in eight and ten days from hog cholera. The 
animals which had received the preventive treatment were 
slightly ill for a few days with swelling at the point, of inocu¬ 
lation, which finally opened and then healed nicely, and with¬ 
in a week the guinea-pigs were well. 
Three weeks after the inoculation one of these animals 
was chloroformed and examined post-mortem. Not the 
slightest scar could be discovered, all the organs appeared 
perfectly normal, and no germs were found. 
Experiment IV.—Four guinea-pigs were treated, two with 
a mixture of sucholotoxins, two with sucholotoxin and albu¬ 
min. The injections were made as before, subcutaneously in 
the thighs, and at intervals extending over a period of four 
weeks. The sore caused by each injection was allowed to 
heal before the next one was made. After the animals had 
recovered from the last treatment two checks were selected, 
and the six were each inoculated with tV c. c. hog cholera 
virus. The checks died, one in eight and the other in ten 
days, the post-mortem examination showing characteristic 
hog cholera lesions. The animals having the preventive 
treatment were ill for about four days, those that received 
