590 
-PAUL GIBIER. 
coccus pyogenes, micro-bacillus prodigiosus, bacillus mega- 
terium and the bacillus osteomyelitis. 
The peroxide of hydrogen which I used was a 3.2 
per cent, solution, jdelding fifteen times its volume of oxygen, 
but this strength was reduced to about 1.5 per cent, corres¬ 
ponding to about eight volumes of oxygen, by adding the 
fresh culture containing the microbe upon which I was ex¬ 
perimenting. I have also experimented upon old cultures 
loaded with a large number of spores of the bacillus anthracis. 
In all cases my experiments were made with a few cubic cen¬ 
timeters of culture in sterilized test-tubes, in order to obtain 
accurate results. 
The destructive action of peroxide of hydrogen, even 
diluted in the above proportions, is almost instantaneous. 
After a contact of a few minutes, I have tried to cultivate the 
microbes which were submitted to the peroxide, but unsuc¬ 
cessfully, owing to the fact that the germs had been complete¬ 
ly destroyed. 
My next experiments were made on the hydrophobic virus 
in the following manner: 
I mixed with sterilized water a small quantity of the 
medulla taken from a rabbit that had died of hydrophobia, 
and to this mixture added a small quantity of peroxide of 
hydrogen. Abundant effervescence took place, and, as soon 
as it ceased, having previously trephined a rabbit, I injected 
a large dose of the mixture under the dura mater. Slight 
effervescence immediately took place and lasted a few 
moments, but the animal was not more disturbed than when 
an injection of the ordinary virus is given. This rabbit is still 
alive, two months after the inoculation. 
A second rabbit was inoculated with the same hydropho¬ 
bic virus which had not been submitted to the action of the 
peroxide, and this animal died at the expiration of the eleventh 
day with the symptoms of hydrophobia. 
I am now experimenting in the same manner upon the bacil¬ 
lus tuberculosis, and if I am not deceived in my expectation, I 
will be able to impart to the profession some interesting 
results. 
It is worthy of notice that water charged, under pressure, 
