PEROXIDE OF HYDROGEN AND OZONE. 591 
with fifteen times its volume of pure oxygen has not the anti¬ 
septic properties of peroxide of hydrogen. This is due to the 
fact that when the peroxide is decomposed nascent oxygen 
separates in that most active and potent of its conditions next 
to the condition, or allotropic form, known as “ ozone.\ 
Therefore it is not illogical to conclude that ozone is the active 
element of peroxide of hydrogen. 
Although peroxide of hydrogen decomposes rapidly in the 
presence of organic substances, I have observed that its de¬ 
composition is checked to some extent by the addition of a 
sufficient quantity of glycerine ; such a mixture, however, 
cannot be kept fora long time, owing to the slow but constant 
formation of secondary products, having irritating properties. 
Before concluding I wish to call attention to a new oxygen¬ 
ated compound, or rather ozonized compound, which has been 
recently discovered and called “ glycozone,” by Mr. Marchand. 
This glycozone results from the reaction which takes place 
when glycerine is exposed to the action of ozone under pres¬ 
sure—one volume of glycerine with fifteen volumes of ozone 
produces glycozone. 
By submitting the bacillus anthracis, pyocyaneous, prodi- 
giosus, and megaterium to the action of glycozone, they were 
almost immediately destroyed. 
I have observed that the action of glycozone upon the 
typhoid fever bacillus, and some other germs, is much slower 
than the influence of peroxide of hydrogen. 
In the dressing of wounds, ulcers, etc., the antiseptic in¬ 
fluence of glycozone is rather slow if compared with that of 
peroxide of hydrogen, with which it may, however, be mixed 
at the time of using. 
It has been demonstrated in Pasteur’s laboratory that gly¬ 
cerine has no appreciable antiseptic influence upon the virus 
of hydrophobia; therefore, I mixed the virus of hydrophobia 
with glycerine, and at the expiration of several weeks all the 
animals which I inoculated with this mixture died with the 
symptoms of hydrophobia. 
On the contrary, when glycerine has been combined with 
ozone to form glycozone, the compound destroys the hydro- 
phobic virus almost instantaneously. 
