634 
KICHAKD MIDDLETON. 
blood appeared dark and coagulated, spleen hyperasmic and 
swollen; in the blood contained in the heart an immense 
number of long oval bacteria were detected ; these were, how¬ 
ever, larger than those found in the spleens of the deceased 
calves, but responded to the stain in the same manner. Two 
rabbits which were inoculated with the blood and spleens of 
these mice died twelve to eighteen hours after; in the heart 
blood, and equally distributed in the vessels of the various 
organs, innumerable bacteria were found. Both showed 
severe hemorrhagic tracheitis ; a pale and swollen spleen ; the 
other abdominal organs, beyond being injected, were normal; 
the blood densely coagulated. From the blood and organs of 
the rabbits and mice, gelatine and agar-gelatine cultures were 
made. 
The calves were removed to another portion of the farm 
and their food changed; the result was, that the affliction en¬ 
tirely disappeared ; there were in all twenty-two deaths. 
Not many days later the same colleague sent me the 
spleens of two calves that had died on another farm on which, 
in the course of a few days, five had succumbed; only two 
post-mortems were held, but these showed large areas of in¬ 
jected pleura, pericardium and peritoneum ; echymoses upon 
the surface of, and parenchymatous degeneration of the heart. 
In both spleens the ovoid bacteria could be observed in 
large quantities ; from this we concluded that in all probability 
we had to do with the same affection as upon the first farm. 
Later 1 received microscopical preparations from the spleen 
of a one-year-old calf that was found cjead in the stall and 
which had previously shown no signs of disease. By dissec¬ 
tion of the same my colleague had found portions which were 
apparently anthracoid; the blood was dark and not firmly 
coagulated; spleen very large, dark and soft; extensive 
ecchymoses upon the peri and endocardium, with numerous 
hemorrhages superficially situated upon the abdominal organs. 
In this case the small ovoid bacteria were also found and 
obtained pure by inoculating mice, and through gelatine 
cultures. 
The quarters and food of these calves, upon the second 
