ORIGINAL ARTICLES. 
RECENT METHODS FOR THE DIAGNOSIS OF GLANDERS.* 
By F. B. Hadley, D.V.M., University of Wisconsin, Madison, Wis. 
This paper has been prepared more for the interest of the 
every-day practitioner than as a strictly scientific contribution. 
It is hoped that the information it presents may help to lessen 
the difficulty of choosing a suitable diagnostic agent from the 
numerous tests which have been devised for the diagnosis of 
glanders in horses. 
With this end in view, a somewhat limited comparative study 
has been undertaken of the recent methods which have been ad¬ 
vised by various scientists as a means for the determination of 
glanders, together with some notes on the harmonization of these 
different methods. 
Bv recent methods is meant those which have come into use 
since the science of bacteriology has developed along pathogenic 
lines, and especially since the serium and toxin diagnosis of dis¬ 
ease has assumed prominence. Each method to which reference 
will be made has been brought forth with the hope that it would 
determine with accuracy whether the horse was glandered or free 
from the infection. All have not fulfilled the hope entertained. 
Three of the methods require the blood serum of the animal 
as a basis for the respective tests. The blood is drawn from the 
jugular vein of the horse by means of a large hypodermic needle 
or a capillary trocar. The instrument should be pointed upward 
when inserted and the blood caught in large-mouth, sterile bottles 
with a capacity of about one ounce. On being allowed to stand 
at room temperature for a few minutes, the blood corpuscles set- 
* Presented to the Wisconsin Society of Veterinary Graduates, January 24, 1912, at 
Madison. 
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